Name of instrument, m RealTime System composed of the msp and mrt modules. Country where designed/Manufactured/Reagents. The Abbott m System comprises the msp and mrz instruments, which are operated with separate System Control Center (SCC). J Clin Microbiol. Jun;47(6) doi: /JCM Epub Apr Performance of the Abbott real-time PCR assay using msp and .

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However, comparison of the differences of VL measurements between the two methods by using the Bland Altman plot showed rough symmetric distribution of the differences and the log differences in It is unclear at the moment the source of the discrepancies. October 02, ; Accepted Date: The fluorescence activity was converted into VL yields by the analyzer Xbbott, Secure We only pass your details on to trusted suppliers at your request.

In addition, their principles in designing primers and probes are also different.

Detection of the resulting fluorescence collectively provides an immediate real-time quantification of the PCR process. The plot to the left represents the distribution of the VL from the Abbott method assay and the plot to the right represents the distribution of VL from the Roche assay. It runs 96 samples per run per day with more if done overnight. Finally, the NA was released using an elution buffer. The Abbott method uses real-time platforms with fluorescence detection systems Abbott, b ; Scott et al.

Abbott Molecular is committed to molecular diagnostics.


Ann Jose ankara escort. This automation enables laboratorians to interface where their skills best contribute to results while minimizing errors and contamination. Pharmaceutical Sciences Journals Ann Jose ankara escort. The general principle for NA extraction is similar to that of the Roche method, i. In addition, different precision of the methods could also potentially explain some of the discordant results.

This study was approved by the Institutional Research Board under the protocol number of H Select your language of interest to view the total content in your interested language.


Box and Whisker plot for the quantifi able plasma samples. Detection by real-time technology used two probes of different lengths: J Antivir Antiretrovir 2: All blood samples were collected in EDTA tubes. Of ND Roche samples, 15 of them Short Communication Open Access. In conclusion, the two methods showed strong correlation in measuring VL within their testing dynamic range.

However, this factor should not be the major contributing factor because a recent surveillance study at the University of Maryland Medical Center showed that about Abbott Molecular’s msp for sample preparation and the mrt for real-time amplification and detection are the cornerstones for this process.

Please leave a message, we will get back you shortly. A total of paired plasma samples were tested in the same day by both methods Table 2. Why request a quote through SelectScience?

Abbott M2000sp Liquid Handler W/ M2000rt PCR

Different from the conventional PCR, an internal probe is added to the detection process, which is an oligo nucleotide with both a fluorescent reporter abbot a fluorescent quencher dye attached.

The fluorescence counts were converted into VL measure by the analyzer Abbott, a ; Abbott, Fast Your inquiry will be delivered straight to the manufacturer. This trend has lead to the development and approval of a number of diagnostic assays For reviews of these assays, see Constantine and Zhao, The best fi t for the regression analysis is indicated by the solid line.

The objective of this study was abboty compare results of HIV-1 VL quantification between the two methods. It should be mentioned that the abbotg diversity of HIV-1 subtypes could present a significant challenge in the accurate VL monitoring of HIV-infected patients who abbtot non-B subtypes Damond et al. HIV viral particles were first lysed and an HIV-1 Quantitation Standard QS Armored RNA molecule was added to each sample, which was followed by a series of processes which involves incubation, introduction of magnetic particles, washing to remove unbound particles and elution of nucleic acid NA at elevated temperatures.


The results showed as. This gene-target based amplification method is based on the determination of the threshold cycle CT when the amplified product is detected for the first time and the PCR is still in its exponential phase Ciotti et al. The solid line in the box indicates the median value m20000sp the ends of the whiskers indicate the minimum and maximum values. Receive your quote directly from the manufacturer.

Results of this study are unable to evaluate the potential differences between these two assays in this regard. These two assays share three common features 1 Additional reduction in the lower limits of detection LOD of HIV-1 RNA m200sp the earlier version; 2 New primers and probes designs for recognition aabbott the different viral subtypes and circulating recombinant forms CRFs ; and 3 Reduction in hand-on time by configuring the test near to full automation.

Abbott Msp Liquid Handler W/ Mrt PCR | eBay

The Abbott method positively identified samples out of with the detectability of Consistently, m0200sp of the distribution and median values of VL between these two methods also showed similar median VL values but a slight wider distribution by the Roche method than the Abbott method was seen Figure 2.

Our data showed that these two methods are essentially indistinguishable with a correlation coefficient of 0. Guidelines Upcoming Special Issues.

An internal control was added into the samples preparation procedure. There were a total of 46 The Version 2 of the m open mode 0. For examples, the Abbott method has been shown to have better detectability of non-B subtypes Geelen et al.