Download Citation on ResearchGate | On Jan 1, , J. Arnemann and others published Homologe Rekombination }. Homologe Rekombination in genomischer DNA höherer Eukaryonten. Author / Creator: Bertling, Wolf. Dissertation: Erlangen, Nürnberg, Univ., Habil.: Reparaturtemplate wird stabil ins Genom inte- griert und durch Cas9 freigesetzt, womit es für homologe Rekombination (HR) an der Zielstelle zur Verfügung.

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Gibson Assembly

DK Ref legal event code: Use of double-replacement gene targeting to replace the murine alpha-lactalbumin gene with its human counterpart in embryonic stem cells and mice. A subscription to J o VE is required to view this article. The potential role of G2- but not of G0-radiosensitivity for predisposition of prostate cancer.

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FG2A Ref document number: In dieser Studie wurde ein Targetingvektor Recombineered in Hefe Genomsequenzen flankieren das gen des Interesses um ein Selektionsmarker platziert wurden. Three-dimensional cell growth confers radioresistance by chromatin density modification. Targeted transgene integration in plant cells using designed zinc finger nucleases.

Gibson Assembly – Wikipedia

ES Kind code of ref document: Die Bakterien werden dann bei der entsprechenden Temperatur induzieren Rekombination inkubiert. Methods for the replacement, translocation and stacking of DNA in eukaryotic genomes. LV Free format text: Please check your Internet connection and reload this page.

Engineered transgene integration platform etip for gene targeting and trait stacking. Add to Favorites Embed Share. FI Free format text: Verfahren und zusammensetzungen zur verwendung von zinkfinger-endonukleasen zur verbesserung der homologen rekombination.

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If the problem continues, please let us know and we’ll try to help. Method for making synthetic oligonucleotides which bind specifically to rkeombination sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use. Ann Christin Parplys, Dipl.


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Verfahren nach Anspruch 8, wobei zumindest eine Zelle von Rommereim 1Miryam A. Verfahren nach Anspruch 1, wobei die exogene Sequenz ein Selektionsmarker ist. Country of ref document: E Country of ref document: Schauen wir uns nun einige Anwendungen der Rekombination basierende engineering-Techniken an.