Article in Ciência Rural 25(2) · January with 4 Reads o herpesvírus suíno (PRV, vírus da doença de Aujeszky) têm sido amplamente utilizadas em vários. doença de Aujeszky em sistema de baculovirus. Régia Maria Feltrin IILaboratório de Sanidade, Embrapa Suínos e Aves, Concórdia, SC, Brasil. IIICentro de. CLONING AND EXPRESSION OF AUJESZKY’S DISEASE VIRUS GLYCOPROTEIN E .. vírus da doença de Aujeszky de surtos em suínos no Estado de Santa.
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The nucleotide sequence amplified in this study corresponds to a bp fragment in the gD gene of the PRV genome The analytical sensitivity of the test was estimated to be 1. Published online Ssuinos 1. Moreover, the viral genomes of a related herpesvirus and other DNA genome porcine viruses as follow: Can J Com Med. The assay proved to be very sensitive due to as little as 1.
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Replication in the respiratory tract, central nervous system and reproductive organs is responsible for pathological changes causing different disorders Induction and inhibition of apoptosis by pseudorabies virus in the trigeminal ganglion during acute infection of swine. The analysis directly from clinical samples from naturally infected animals proved the potential usefulness of the method for a rapid disease diagnosis from field cases.
The etiological agent of this disease is suid herpesvirus type 1, usually named pseudorabies virus PRVa pantropic alphaherpesvirus which causes fatal infections in baby pigs, respiratory disease and poor growth in fattening pigs and reproductive disorders in adults 28 The polymerase chain reaction PCR can be used to identify PRV genomes in secretions or organ samples and although some PCR assays for PRV detection with different sensitivities have been reported 37915 there is no standard procedure recommended so far 2.
The assay specificity was demonstrated by the absence of amplifications in all heterologous viruses evaluated and in tissue samples derived from seven healthy pigs. All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License.
Diagnostic methods for detection of Classical swine fever virus—Status quo and new developments. In addition, positive amplifications were obtained in all the tissue samples, from PRV natural infected pigs, evaluated. This assay was based on the amplification of a highly conserved viral gD gene fragment.
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Doença de Aujeszky
The trigeminal ganglion is the most consistent site for virus isolation, although latent virus is usually difficult to culture or even impossible 113 and PCR is the method recommended to detect viral genome present in this site. Oligonucleotide primers and restriction endonuclease selection PRV specific primers were designed using the Oligo 6.
Thus, the optimal concentration of MgCl2 and primers were 1. The viral agent following a primary replication can establish latent infection and develops a latency-reactivation infection which allows its perpetuation in pig populations 1012 In general, PRV infections must be considered in the differential diagnosis of respiratory, reproductive and doeena disorders.
Seven virus-negative tissues samples from clinically healthy animals were also included. Primers sequences, genome positions and the size of PCR products are shown in Table 1.
Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome. Negative controls were run with each test.
Under typical conditions of intensive swine production, several clinically similar viral diseases can occur which require laboratory differential diagnosis. The analytical sensitivity of the test was consistently aujfszky to be 1.
Articles siinos Brazilian Journal of Microbiology are provided here courtesy of Elsevier. Especially HVB1 is an important target for specificity assay because is a related herpesvirus which is known to infect swine BHV-1 4. The virus primarily replicates in the respiratory tract, spreads along cranial nerves to the brains and via lymph and blood to internal organs, with the reproductive organs being affected.
Also, the BLAST search against nucleotide databases of different herpesvirus and random nucleotide sequences revealed this region is very specific for PRV genomes. Agarose gel electrophoresis was used to detect PCR products. Table 1 Primers designed for the specific amplification of the viral gD glycoprotein gene of the PRV genome.
Doença de Aujeszky – Wikipédia, a enciclopédia livre
National Center for Biotechnology InformationU. The development, optimization and evaluation of a polymerase chain reaction PCR assay are presented for the diagnosis of pseudorabies infection. The polymerase chain reaction PCR is a rapid tool that can be used no only to detect acutely PRV infected pigs but it is the recommended test for detect PRV latent infection.