40818 DATASHEET PDF

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Detection of Proteins Directions for Use: Changing to another country might result in loss of shopping cart. Protein Blotting A general protocol for daasheet preparation. Analyze cells in DNA staining solution on flow cytometer.

Preparing Cell Lysates Aspirate media. Electrotransfer to nitrocellulose membrane To prepare 10 ml, add 0. More about how we get our images. USP8 Antibody – Incubate for 30 min at room temperature. To Purchase S View sizes. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.

Solutions and Reagents Achieve higher quality immunofluorescent images using the efficient and cost-effective, pre-made reagents in our Immunofluorescence Application Solutions Kit NOTE: Achieve higher quality immunofluorescent images using the efficient and cost-effective, pre-made reagents in our Immunofluorescence Application Solutions Kit. Remove buffer once solution is clear.

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Pre-wash magnetic beads just prior to use:. Please refer to primary antibody datasheet or product webpage for recommended antibody dilution.

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Proceed with Immunostaining Section C. Pre-wash magnetic beads see Cell Lysate Pre-Clearing datasneet, steps 1 and 2. Polyclonal antibodies are produced by immunizing animals with a synthetic datasjeet corresponding to residues surrounding Pro of human USP8 protein. The optimal lysate concentration will depend on the expression level of the protein of interest. Proceed with detection Section D. Incubate with rotation for 20 min at room temperature. Dilute to 1X with dH 2 O.

Place the tube in a magnetic separation rack for seconds.

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To harvest cells under nondenaturing conditions, remove media and rinse cells once with ice-cold 1X PBS. Incubate with datashedt for 20 minutes at room temperature. Blotting Membrane and Paper: Pre-clear enough lysate for test samples and isotype controls. Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser of dtaasheet STING protein.

Appropriate isotype controls are highly recommended in order to show specific binding in your primary antibody immunoprecipitation. Recommended Fluorochrome-conjugated Anti-Rabbit secondary antibodies: Treat cells by adding fresh media containing regulator for desired time.

The supernatant is the sample. Wash cells by centrifugation in excess 1X PBS to remove methanol. Sample Analysis Proceed to one of the following specific set dstasheet steps. If using whole blood, lyse red blood cells and wash by centrifugation prior to fixation. Aliquot desired number of cells into tubes or wells.

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Antibodies are purified by protein A and peptide affinity chromatography. Pre-wash magnetic beads just prior to use: Ubiquitinating enzymes UBEs catalyze protein ubiquitination, a reversible process countered by deubiquitinating enzyme DUB action 1,2. Recommended Anti-Rabbit secondary antibodies: Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. Allow cells to fix for 15 min at room temperature.

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Transfer the lysate and antibody immunocomplex solution to the tube containing the pre-washed magnetic bead pellet. Vortex, then microcentrifuge for 30 sec.

Collect cells by centrifugation and aspirate supernatant. Alexa Fluor is a registered trademark of Life Technologies Corporation. Count cells using a hemocytometer or alternative method.

Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818

A cell lysate pre-clearing step is highly recommended to reduce non-specific protein binding to the Protein A Magnetic beads. Aspirate fixative, rinse three times in 1X PBS for 5 min each. Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing so that signal attributed to binding datasheeet the new antibody is not leftover signal from the first immunoblotting experiment.